"Justin was solo again at the Saturday Night Live after party and he stayed until nearly 5 a.m.," a witness told Radar Online. "Everyone was buzzing about her absence."
And Biel did not just miss the afterparty. She did not even support her husband during SNL rehearsals on Friday.
"And it's not that Justin was flirting with other women or anything at the party but something just didn't seem right," the source continued. "It was literally what everyone was talking about; whether he and Jessica have split."
But there may be one sign that Justin Timberlake and Jessica Biel are not heading for a split. The singer reportedly wore his wedding band throughout the party. This was a far cry from some of his concerts earlier this month, when he made headlines for leaving his ring off while performing.
Still, this is not the first time Justin Timberlake has attended a major gig solo. Jessica Biel also stayed home for her husband's performance at the American Music Awards last month. But the actress temporarily put a stop to the split rumor that night, tweeting that she was watching the awards show from home.
If Jessica Biel and Justin Timberlake are close to a split, they are certainly keeping the news quiet. Earlier this month, Biel expressed her desire to start a family with her husband.
"I'm not sure about sooner or later," she told People. "But definitely at some point."
For his part, Justin Timberlake gushed about Jessica Biel in March, implying that his wife inspired his hit song "Mirrors".
'"Mirrors' is a love song to someone that you feel like is, you know, sort of your other half," he said. "And it became a play on that. Not specifically about looking into a mirror. One of the most valuable things in a relationship is being able to constantly change and be individual, but look to the other side to the person that you're with and know that they're changing as well individually, but somehow you two can mirror each other and be the other half of that world that you both create."
Based on this quote, it does not sound like Justin Timberlake is ready to split from Jessica Biel. And there may be a logical explanation for the actress' SNL absence this weekend. But Hollywood is full of shocking celebrity breakups. So it would not surprise us if this seemingly happy couple called it quits next year.